| Cat. No. HS-484 017 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Clone | SY-171D8E8 |
| Subtype | IgG2b (λ light chain) |
| Immunogen | synthetic peptide corresponding to residues near the carboxy terminus of mouse iNOS (UniProt Id: P29477) |
| Reactivity |
Reacts with: mouse (P29477). No signal: rat, human. Other species not tested yet. |
| Data sheet | hs-484_017.pdf |
iNOS positive tumor associated macrophages (TAMs) in Cloudman S91 syngeneic mouse tumor
Inducible nitric oxide synthase (iNOS) belongs together with endothelial NOS (eNOS) and neuronal NOS (nNOS) to the NOS family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. In contrast to the constitutively expressed, calcium-dependent eNOS and nNOS isoforms, iNOS is calcium-independent and usually activated in defense responses such as infection and inflammation (1). Expression of the iNOS gene is induced in vitro in macrophages polarized to the pro-inflammatory M1 state (2). NO-producing tumoricidal macrophages in the tumor microenvironment can modulate immune responses and promote apoptosis of tumor cells (2). In the brain, iNOS is normally not expressed except in chronic and acute inflammatory conditions. In ischemic brain injury, iNOS was found to be highly expressed in neutrophils and endothelium (3) and in M1-like pro-inflammatory microglia (4).