iNOS antibody mouse specific - HS-484 017

iNOS is an inducible nitric oxide synthase expressed in inflammatory conditions
Rat monoclonal purified IgG
Cat. No.: HS-484 017
Amount: 100 µg
Price: $415.00
Cat. No. HS-484 017 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: 1 : 200 up to 1 : 1000 gallery  
Clone SY-171D8E8
Subtype IgG2b (λ light chain)
Immunogen synthetic peptide corresponding to residues near the carboxy terminus of mouse iNOS (UniProt Id: P29477)
Reactivity Reacts with: mouse (P29477).
No signal: rat, human.
Other species not tested yet.
Data sheet hs-484_017.pdf
Cat. No.: HS-484 017
Amount: 100 µg
Price: $415.00

iNOS positive tumor associated macrophages (TAMs) in Cloudman S91 syngeneic mouse tumor


Inducible nitric oxide synthase (iNOS) belongs together with endothelial NOS (eNOS) and neuronal NOS (nNOS) to the NOS family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. In contrast to the constitutively expressed, calcium-dependent eNOS and nNOS isoforms, iNOS is calcium-independent and usually activated in defense responses such as infection and inflammation (1). Expression of the iNOS gene is induced in vitro in macrophages polarized to the pro-inflammatory M1 state (2). NO-producing tumoricidal macrophages in the tumor microenvironment can modulate immune responses and promote apoptosis of tumor cells (2). In the brain, iNOS is normally not expressed except in chronic and acute inflammatory conditions. In ischemic brain injury, iNOS was found to be highly expressed in neutrophils and endothelium (3) and in M1-like pro-inflammatory microglia (4).

Protocols for HS-484 017