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T-bet antibody mouse specific - HS-487 008

T-bet is a transcription factor expressed by several lymphocyte lineages
Rabbit monoclonal recombinant IgG
Cat. No.: HS-487 008
Amount: 50 µg
Price: $415.00
Cat. No. HS-487 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: 1 : 200 (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone Rb87E12
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues surrounding AA 470 of mouse T-bet (UniProt Id: Q9JKD8)
Reactivity Reacts with: mouse (Q9JKD8).
No signal: human (Q9UL17), rat.
Other species not tested yet.
Remarks

This antibody is a chimeric antibody based on the monoclonal rat antibody clone SY-87E12. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC-P: Heat-mediated antigen retrieval (Tris/EDTA pH 9.0) is recommended for immunohistochemical staining. The antibody shows some unspecific staining in mouse cerebellum.
Data sheet hs-487_008.pdf
Cat. No.: HS-487 008
Amount: 50 µg
Price: $415.00

Nuclear T-bet/Tbx-21 expression (red) is detected in CD4-positive T cells (green) in a Toxoplasma (T.) gondii infected mouse liver section

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Background

T-bet (also known as Tbx21) belongs to the T-box subfamily Tbr1, and is an important transcription factor for the immune system. T-bet is a potent transactivator of the IFNγ gene and its expression correlates with IFNγ expression in Th1 cells, NK cells, and B cells (1). In CD4 T cells, Th1 and Th2 lineage commitment is regulated by opposing actions of T-bet and GATA-3 (2). T-bet promotes CD4 Th1 differentiation and represses alternative cell fates like Th2, or Th17, whereas GATA-3 is the lineage determining transcription factor for Th2 cells (2). CD8+ T cells require T-bet expression to differentiate into effector cells (3). In NK cells, T-bet expression is required for NK cell maturation (4).
Protocols for HS-487 008
IHC-P
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