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HNK-1 (CD57) antibody - HS-531 008

HNK-1 is a sulfate carbohydrate epitope
Rabbit monoclonal recombinant IgG
Cat. No.: HS-531 008
Amount: 50 µg
Price: $420.00
Cat. No. HS-531 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC-P: 1 : 400 up to 1 : 1000 gallery  
ELISA: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone Rb-HNK-1
Subtype IgG1 (κ light chain)
Immunogen Membrane extract of human lymphoblastoid cell line HSB-2 (UniProt Id: Q9P2W7)
Reactivity Reacts with: human (Q9P2W7).
No signal: mouse, rat.
Other species not tested yet.
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone HNK-1. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet hs-531_008.pdf
Cat. No.: HS-531 008
Amount: 50 µg
Price: $420.00

HNK-1 / CD57 positive cells are located in the germinal centers of the human tonsil

IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
Background

The human natural killer-1 (HNK-1) glyco-epitope, also known as LEU-7 or CD57, was first identified by a monoclonal antibody that recognizes a subset of human natural killer (NK) cells (1). This epitope features a unique trisaccharide structure, consisting of a 3-O-sulfated glucuronic acid linked to N-acetyl-lactosamine (HSO3-3GlcAß1-3Galß1-4GlcNAc-) and is primarily expressed on glycolipids and glycoproteins within the nervous system. Notable carriers are NCAM, L1, MAG, tenascin-R and tenascin-C (2). Studies in mice lacking HNK-1 expression reveal abnormal brain function, including impaired synaptic plasticity, deficits in dendritic spine maturation and disrupted spatial learning (2-4). In humans, HNK-1 is implicated in various central nervous system (CNS) disorders. For instance, autoantibodies targeting HNK-1 have been detected in certain neuropathies (5). Beyond its role in neural development, HNK-1 also appears to function as tumor suppressor. Elevated HNK-1 expression has been associated with improved survival outcomes in patients with prostate cancer or astrocytic tumors (6,7). In the field of immunology, HNK-1 is most commonly referred to as CD57. It is expressed on a subset of immune cells, particularly T cells and natural killer (NK) cells, and is associated with terminal differentiation and cellular senescence (8).
Protocols for HS-531 008
IHC-P