| Cat. No. HS-532 003 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Synthetic peptide corresponding to residues near the carboxy terminus of mouse ICAM1 (UniProt Id: P13597) |
| Reactivity |
Reacts with: mouse (P13597). Weaker signal: rat (Q00238). No signal: human (P05362). Other species not tested yet. |
| Remarks |
IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) is required for immunohistochemical staining. |
| Data sheet | hs-532_003.pdf |
ICAM1 is constitutively expressed on alveolar epithelial cells (red) of the mouse lung, among which are embedded LAMP3-positive pneumocytes II (green).
Intercellular Adhesion Molecule 1 (ICAM1), also known as CD54 and referred to as MALA-2 in mice (1), is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed at low levels under physiological conditions on endothelial, epithelial, and immune cells. However, its expression is markedly upregulated in response to pro-inflammatory cytokines such as TNFα, IL-1β, and IFNγ (2). ICAM1 is best known for its critical role in leukocyte transmigration across the vascular endothelium into sites of inflammation. This process primarily depends on its interaction with leukocyte counterreceptors, namely lymphocyte function-associated antigen-1 (LFA-1) and macrophage antigen-1 (Mac-1) (3).
Beyond facilitating leukocyte trafficking, ICAM1 also serves as an efferocytosis receptor in inflammatory macrophages (4) and contributes to the formation of immune synapses (5).
Upon endothelial activation, ICAM1 expression is strongly induced, and a soluble form (sICAM1) is released because of proteolytic cleavage (6). Elevated levels of sICAM1 have been detected in the sera of patients with various types of cancer and are associated with poor prognostic outcomes (7).