HistoSure - Aquaporin4

Cat. No.: 429 006

Amount: 50 µg

Price: $385.00

Cat. No. 429 006	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 1000 up to 1 : 2000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 249 to 323 from mouse Aquaporin-4 isoform 2 (UniProt Id: P55088-1)
Reactivity	Reacts with: rat (P47863), mouse (P55088-1). Other species not tested yet.
Specificity	Specific for Aquaporin-4, detects all described isoforms.
Matching control protein/peptide	429-0P
Remarks	IHC: Antigen retrieval with citrate buffer pH 6 is tolerated.
Data sheet	429_006.pdf

References for Aquaporin4 - 429 006

Inhibiting astrocyte connexin-43 hemichannels blocks radiation-induced vesicular VEGF-A release and blood-brain barrier dysfunction.
Schumacher S, Tahiri H, Ezan P, Rouach N, Witschas K, Leybaert L
Glia (2024) 721: 34-50. 429 006 IHC; tested species: mouse

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 429 006 IHC; tested species: mouse

Immunotherapy targeting plasma ASM is protective in a mouse model of Alzheimer's disease.
Choi BJ, Park MH, Park KH, Han WH, Yoon HJ, Jung HY, Hong JY, Chowdhury MR, Kim KY, Lee J, Song IS, et al.
Nature communications (2023) 141: 1631. 429 006 IHC; tested species: mouse

Evaluation of gliovascular functions of Aqp4 readthrough isoforms.
Mueller SM, White KM, Fass SB, Chen S, Shi Z, Ge X, Engelbach JA, Gaines SH, Bice AR, Vasek MJ, Garbow JR, et al.
bioRxiv : the preprint server for biology (2023) : . 429 006 IHC; tested species: mouse

Cat. No.: 429 006

Amount: 50 µg

Price: $385.00

ICC

IHC

Background

Aquaporin4, or AQP4, also known as Mercurial-insensitive water channel (MIWC), is a member of the aquaporin water channel family. This osmoreceptor regulates body water balance and mediates water flow within the central nervous system.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Mack AF, Brain structure & function (2018) : .
2 ) Amann B, International journal of molecular sciences (2016) 177: .
3 ) Nagelhus EA, Physiological reviews (2013) 934: 1543-62.

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Aquaporin4 antibody - 429 006

References for Aquaporin4 - 429 006