Aquaporin4 antibody - 429 009

Aquaporin4 is a water-specific channel in the blood brain barrier
Chicken monoclonal recombinant IgY
Cat. No.: 429 009
Amount: 50 µg
Price: $415.00
Cat. No. 429 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: yes
ICC: 1 : 1000 up to 1 : 2000 gallery  
IHC: 1 : 2000 up to 1 : 4000 gallery  
IHC-P: 1 : 1000 up to 1 : 2000 gallery  
IHC-Fr: 1 : 1000 up to 1 : 2000 (see remarks) gallery  
Clone Ch325B5A5
Subtype IgY (λ light chain)
Immunogen Recombinant protein corresponding to AA 249 to 323 from mouse Aquaporin4 isoform 2 (UniProt Id: P55088-1)
Reactivity Reacts with: mouse (P55088-1), rat (P47863), human (P55087).
Other species not tested yet.
Matching control protein/peptide 429-0P
Remarks

This antibody is a chimeric antibody based on the well-known monoclonal mouse antibody clone 325B5A5. The constant regions of the heavy and light chains have been replaced with chicken-specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
IHC-Fr: Methanol fixation is recommended.

Data sheet 429_009.pdf
Cat. No.: 429 009
Amount: 50 µg
Price: $415.00
Background

Aquaporin4, or AQP4, also known as Mercurial-insensitive water channel (MIWC), is a member of the aquaporin water channel family. This osmoreceptor regulates body water balance and mediates water flow within the central nervous system.