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Aquaporin4 antibody - 429 011BT

Aquaporin4 is a water-specific channel in the blood brain barrier
Mouse monoclonal purified IgG
Cat. No.: 429 011BT
Amount: 100 µg
Price: $485.00
Cat. No. 429 011BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.
Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 1000 up to 1 : 2000 gallery  

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label biotin
Clone 325B5A5
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 249 to 323 from mouse Aquaporin-4 isoform 2 (UniProt Id: P55088-1)
Reactivity Reacts with: mouse (P55088-1), rat (P47863), human (P55087).
Other species not tested yet.
Matching control protein/peptide 429-0P
Data sheet 429_011bt.pdf
Cat. No.: 429 011BT
Amount: 100 µg
Price: $485.00
ICC
ICC
IHC
IHC
IHC
IHC-P
IHC-P
IHC-P
Background

Aquaporin4, or AQP4, also known as Mercurial-insensitive water channel (MIWC), is a member of the aquaporin water channel family. This osmoreceptor regulates body water balance and mediates water flow within the central nervous system.

 

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.
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