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This rabbit polyclonal antibody is specifically developed for mouse E-cadherin (e.g., it does not recognize the human E-cadherin protein) and is superior for detection of murine E-cadherin in WB, IHC and IHC-P (FFPE) applications. Epithelial cadherin (E-cadherin) also known as Cadherin-1, CAM 120/80 or uvomorulin belongs, together with neuronal (N) cadherin, to the type I classical cadherins, transmembrane proteins that function in calcium-dependent cell-cell adhesion (1).
In normal tissues, E-cadherin is expressed by most epithelial cells. A distinct distribution of E-cadherin expression is found in the kidneys, where only distal tubuli show E-cadherin expression and in the placenta, where only the cytotrophoblastic layer stains positive for E-cadherin (2). In the human normal adult nervous system E-cadherin expression is limited to the arachnoid membrane, whereas in mice E-cadherin is also expressed in neural stem cells, where E-cadherin regulates self-renewal (3).
Figure 1: Indirect immunostaining staining of formalin fixed paraffin embedded murine tissue sections (A: mouse appendix; B: mouse liver; C: mouse kidney; D: mouse lung) with rabbit anti-E-cadherin (cat. no. HS-467 003, 1:200; DAB). Nuclei have been visualized by haematoxylin staining (blue).
Figure 2: Indirect immunostaining of human and mouse duodenum section using mouse-specific rabbit polyclonal anti-E-cadherin (cat. no. HS-467 003, 1:200; DAB). The antibody is not cross-reacting with human E-cadherin. Nuclei have been visualized by haematoxylin staining (blue).
E-cadherin is a potent tumor suppressor and the so-called “cadherin switch” - downregulation of E-cadherin while N-cadherin is upregulated - is often found in malignant epithelial cancers. This Epithelial-to-Mesenchymal Transition (EMT) has been shown to be crucial in tumorigenesis where the EMT program enhances metastasis, chemoresistance and tumor stemness (4).
However, E-cadherin upregulation in malignancies derived from E-cadherin negative normal tissues tend to be linked to unfavorable tumor phenotype and disease outcome (2). In syngeneic mouse tumors E-cadherin expression is typically lower than in human tumors suggesting that syngeneic mouse models have a more mesenchymal-like tumor cellular phenotype (5).
| Cat. No. | Product Description | Application | Quantity | Price | Cart |
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HS-467 003 | E-cadherin, rabbit, polyclonal, affinity purifiedaffinity mouse specific | WB IHC IHC-P | 50 µg | $370.00 |   |
Halbleib, Nelson 2006: Cadherins in development: cell adhesion, sorting, and tissue morphogenesis.
PMID: 17158740
Burandt, et al. 2021: E-Cadherin expression in human tumors: a tissue microarray study on 10,851 tumors.
PMID: 34090526
Lewis-Tuffin, et al. 2010: Misregulated E-cadherin expression associated with an aggressive brain tumor phenotype. PMID: 21060868
Loh, et al. 2019: The E-Cadherin and N-Cadherin Switch in Epithelial-to-Mesenchymal Transition: Signaling, Therapeutic Implications, and Challenges. PMID: 31547193
Zhong et al. 2020: Comparison of the molecular and cellular phenotypes of common mouse syngeneic models with human tumors. PMID: 31898484
