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CD11c peptide - 375-0P

CD11c is a fibrinogen receptor involved in cell-cell interaction during inflammation
control peptide
Cat. No.: 375-0P
Amount: 100 µg
Price: $105.00
Cat. No. 375-0P 100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Control peptides should be stored at -20°C when still lyophilized!
Applications
 
WB: N/A
IP: N/A
ICC: N/A
IHC: N/A
IHC-P: N/A

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 1159 to 1169 from mouse CD11c (UniProt Id: Q9QXH4)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies HS-375 003, HS-375 004
Remarks

This control peptide consists of the synthetic peptide (aa 1159 - 1169 of mouse CD 11c) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet 375-0p.pdf
Cat. No.: 375-0P
Amount: 100 µg
Price: $105.00
Background

CD11c or Integrin alpha-X (ITGAX) is a heterodimeric glycoprotein consisting of an α- and β-subunit and has seven repeating integrin domains. This transmembrane receptor type I plays a pivotal role in T cell killing and mediates intercellular adhesions during inflammation. Predominat expression levels have been found in dendritic cells, monocytes, macrophages, neutrophils and a small subset of B cells. Under pathological conditions, CD11c is a marker for hairy cell leukemia, acute non-lymphocytic leukemias, and some chronic lymphocytic leukemias.
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