Home|New Products||||||Technical support|
Featured Product

Featured product: rat anti-ms CD3e (Cat. HS-413 117)

The T‐cell receptor (TCR)–CD3 complex, expressed on T cells, is responsible for the recognition of antigens bound to major histocompatibility complex (MHC) molecules and determines the outcome of T-cell responses. It helps to activate both the CD8+ cytotoxic T cells and the CD4+ T helper cells.

Each T cell contains a unique αβ T-cell receptor (TCR), which does not possess intracellular signaling domains itself. Instead the TCR is noncovalently associated with a multisubunit signaling apparatus, consisting of CD3γ, CD3δ chain, CD3ε chains and CD3ζζ-chains.

TCR/CD3 signaling is central to the initiation of antigen-specific T cell responses to pathogens and vaccines, as well as transplanted tissues, tumors, and autoantigens. CD3 is initially expressed in the cytoplasm of pro-thymocytes. During T cell maturation the expression of CD3 migrates to the cell-membrane. The specific appearance at all stages of T cell development make CD3 a useful immunohistochemical marker for T cells in tissue sections.

In the clinical setting, CD3 is a relevant marker for the classification of malignant lymphomas and leukemias as the antigen remains present in almost all T-cell lymphomas and leukemias. It can also be used to detect T cells in celiac disease, lymphocytic and collagenous colitis.

 

The HistoSure rat anti-mouse CD3e antibody has been generated against an epitope unique for mouse CD3e. Specificity for mouse CD3e has been tested in a broad panel of mouse and human tissues. No cross-reactivity was detected with human CD3e. Therefore, it is a unique tool to study the incidence of leaky T cells in humanized mice engrafted with human bone marrow, cord blood, or G-CSF cytokine-mobilized peripheral blood mononuclear cells. Leakiness, in which T and B cells develop in aging mice, occurs for example at high frequency in the immunodeficient mouse strains SCID and NOD/SCID (Bosma, 1992).

anti-mouse CD3e in mouse spleen and human tonsil

Figure: Immunohistochemical staining of FFPE mouse spleen and human tonsil using rat anti-mouse CD3e (cat. no. HS-413 117). Nuclei have been counterstained with haematoxylin (blue).

The HistoSure rat anti-mouse CD3e antibody has been generated against an epitope unique for mouse CD3e. Specificity for mouse CD3e has been tested in a broad panel of mouse and human tissues. No cross-reactivity was detected with human CD3e. Therefore, it is a unique tool to study the incidence of leaky T cells in humanized mice engrafted with human bone marrow, cord blood, or G-CSF cytokine-mobilized peripheral blood mononuclear cells. Leakiness, in which T and B cells develop in aging mice, occurs for example at high frequency in the immunodeficient mouse strains SCID and NOD/SCID (Bosma, 1992).

Figure: Immunohistochemical staining of FFPE mouse spleen and human tonsil using rat anti-mouse CD3e (cat. no. HS-413 117). Nuclei have been counterstained with haematoxylin (blue).

 

The HistoSure rat anti-mouse CD3e antibody has been specifically developed for use in formalin fixed paraffin embedded (FFPE) tissue sections. Therefore, it is a powerful tool to study T cell biology in preclinical mouse models, such as preclinical mouse cancer models.

Infiltration of CD3e positive cells in murine breast tumor
Doublestaining of CD19 and CD3e in mouse thymus
Figure: Staining for anti-mouse CD3e (cat. no. HS-413 117) in FFPE breast cancer model was performed on a Dako Autostainer Courtesy: Discovery Services, Charles River, Freiburg, Germany.
Figure: Immunohistochemical doublestaining of formalin-fixed paraffin-embedded mouse thymus using rabbit anti-mouse CD19 (cat. no. HS-439 003; AP-RED, red color) and rat anti-mouse CD3e (cat. no. HS-413 117; DAB, brown color). Nuclei have been counterstained with haematoxylin (blue).
  • Bosma, 1992: B and T Cell Leakiness in the Scid Mouse Mutant. PMID: 1449786