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The T‐cell receptor (TCR)–CD3 complex, expressed on T cells, is responsible for the recognition of antigens bound to major histocompatibility complex (MHC) molecules and determines the outcome of T-cell responses. It helps to activate both the CD8+ cytotoxic T cells and the CD4+ T helper cells.
Each T cell contains a unique αβ T-cell receptor (TCR), which does not possess intracellular signaling domains itself. Instead the TCR is noncovalently associated with a multisubunit signaling apparatus, consisting of CD3γ, CD3δ chain, CD3ε chains and CD3ζζ-chains.
TCR/CD3 signaling is central to the initiation of antigen-specific T cell responses to pathogens and vaccines, as well as transplanted tissues, tumors, and autoantigens. CD3 is initially expressed in the cytoplasm of pro-thymocytes. During T cell maturation the expression of CD3 migrates to the cell-membrane. The specific appearance at all stages of T cell development make CD3 a useful immunohistochemical marker for T cells in tissue sections.
In the clinical setting, CD3 is a relevant marker for the classification of malignant lymphomas and leukemias as the antigen remains present in almost all T-cell lymphomas and leukemias. It can also be used to detect T cells in celiac disease, lymphocytic and collagenous colitis.
The HistoSure rat anti-mouse CD3e antibody has been generated against an epitope unique for mouse CD3e. Specificity for mouse CD3e has been tested in a broad panel of mouse and human tissues. No cross-reactivity was detected with human CD3e. Therefore, it is a unique tool to study the incidence of leaky T cells in humanized mice engrafted with human bone marrow, cord blood, or G-CSF cytokine-mobilized peripheral blood mononuclear cells. Leakiness, in which T and B cells develop in aging mice, occurs for example at high frequency in the immunodeficient mouse strains SCID and NOD/SCID (Bosma, 1992).
Figure 1: Immunohistochemical staining of FFPE mouse spleen and human tonsil using rat anti-mouse CD3e (cat. no. HS-413 117). Nuclei have been counterstained with haematoxylin (blue).
The HistoSure rat anti-mouse CD3e antibody has been generated against an epitope unique for mouse CD3e. Specificity for mouse CD3e has been tested in a broad panel of mouse and human tissues. No cross-reactivity was detected with human CD3e. Therefore, it is a unique tool to study the incidence of leaky T cells in humanized mice engrafted with human bone marrow, cord blood, or G-CSF cytokine-mobilized peripheral blood mononuclear cells. Leakiness, in which T and B cells develop in aging mice, occurs for example at high frequency in the immunodeficient mouse strains SCID and NOD/SCID (Bosma, 1992).
Figure 1: Immunohistochemical staining of FFPE mouse spleen and human tonsil using rat anti-mouse CD3e (cat. no. HS-413 117). Nuclei have been counterstained with haematoxylin (blue).
The HistoSure rat anti-mouse CD3e antibody has been specifically developed for use in formalin fixed paraffin embedded (FFPE) tissue sections. Therefore, it is a powerful tool to study T cell biology in preclinical mouse models, such as preclinical mouse cancer models.
Figure 2: Staining for anti-mouse CD3e (cat. no. HS-413 117) in FFPE breast cancer model was performed on a Dako Autostainer Courtesy: Discovery Services, Charles River, Freiburg, Germany.
Figure 3: Immunohistochemical doublestaining of formalin-fixed paraffin-embedded mouse thymus using rabbit anti-mouse CD19 (cat. no. HS-439 003; AP-RED, red color) and rat anti-mouse CD3e (cat. no. HS-413 117; DAB, brown color). Nuclei have been counterstained with haematoxylin (blue).
| Cat. No. | Product Description | Application | Quantity | Price | Cart |
|---|
HS-439 003 | CD19, rabbit, polyclonal, affinity purifiedaffinity mouse specific | WB ICC IHC IHC-P | 200 µl | $370.00 |   |
| HS-413 117 | CD3e, rat, monoclonal, purified IgG IgG mouse specific | IHC-P | 200 µl | $415.00 | |