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Incubation of primary antibody
Fluorescent and chromogenic Detection
Validation of HistoSure antibodies
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> IHC-P Guidelines
Reference protocols HistoSure
Where can I find Reference protocols for my HistoSure products?
Protease-mediated and heat-mediated Antigen Retrieval
Antigen Retrieval is an essential step in IHC-P staining protocols. Overview on basic principles and essential techniques of Antigen Retrieval.
Primary antibody incubation instructions
Why do we recommend incubation of primary antibodies for 60 minutes at room temperature?
Primary antibody detection
Visualization of target antigen: overview on direct and indirect methods for primary antibody detection.
Fluorescent and Chromogenic Immunohistochemistry
Principles of fluorescent and chromogenic immunohistochemistry.
Differentiate between false positive staining and true signals: Overview on essential and recommended controls in IHC-P.
Overview on how to use Multiplex IHC-P for detection of more than one target protein.
Optimize your staining results using our IHC-P Guidelines. For further questions please contact our Technical support.