Irreproducibilty of results is a major issue in the scientific community. Numerous factors influencing the failure to reproduce other's experiments have been identified. One issue is the lack of original protocols.
We strive for maximum transparency. Therefore we provide a Reference Protocol for every HistoSure product application we tested in-house. However, to achieve the highest specific signal and lowest non-specific background signal, customers may adapt experimental conditions individually. Please also refer to our IHC-P Guidelines about antigen retrieval, primary antibody incubation and detection techniques and to our general protocols on the Synaptic Systems main page.