Immunohistochemical doublestaining for macrophage markers in formalin fixed paraffin-embedded human tonsil sections. Tissue were stained with rat anti-human CD68 (cat. no. HS-460 017, dilution 1:100; red) and rabbit anti-mouse F4/80 (cat. no. HS-397 008, dilution 1:100; green). Nuclei have been counterstained with DAPI (blue).
Immunohistochemical staining of formalin fixed paraffin-embedded sections of a human lung with rat monoclonal anti-CD11b (cat. no. HS-384 017, dilution 1:100; DAB). The antibody is specific for the human protein and does not cross-react with murine cells. Nuclei have been counterstained with haematoxylin (blue).
Indirect immunostaining of PFA fixed murine spleen section with polyclonal rabbit anti-CD163 (cat. no.: HS-455 003, dilution 2 µg/ml; red) and polyclonal Guinea pig anti-IBA1 (cat. no.: HS-234 004, dilution 1:500; green). CD163 is expressed in a subpopulation of red pulp macrophages in the spleen. Nuclei have been visualized by DAPI staining (blue).
Immunohistochemical staining of murine formalin fixed paraffin-embedded spleen with monoclonal rat anti-IBA1 (cat. no.: HS-234 017, dilution 1:100; DAB) and rabbit monoclonal anti-F4/80 (cat. no.: HS-397 008, dilution 1:100; AP-RED). Nuclei have been counterstained with haematoxylin.
Immunohistochemical staining of formalin fixed paraffin-embedded mouse colon section with monoclonal rabbit anti-IBA1 (cat. no.: HS-234 008, dilution 1:500; DAB). Nuclei have been counterstained with haematoxylin (blue). Staining was performed with a Thermo Scientific Autostainer.
Immunohistochemical staining of formalin fixed paraffin-embedded of (A) a CK7-positive human pancreatic adenocarcinoma and (B) a CK7-negative human colon carcinoma engrafted into immunocompromised mice (xenograft) with rat monoclonal anti-human CK7 (cat. no.: HS-454 017, dilution 1:100; DAB). This antibody is fully human-specific and does not stain murine CK7-positive cells. Nuclei have been counterstained with haematoxylin (blue).
Immunohistochemical staining of formalin fixed paraffin-embedded human tonsil section (left) and mouse spleen section (right) with rat monoclonal anti-human CD3e (cat. no.: HS-413 017, dilution 1:200; DAB). This antibody is suited to distinguish human from murine T-cells in humanized mouse models. Nuclei have been counterstained with haematoxylin (blue).
Immunohistochemical staining of formalin fixed paraffin-embedded brain sections from a non-infected PBS control mouse and a virus infected mouse with monoclonal rat anti-CD11b (cat. no.: HS-384 117, dilution 1:200; DAB). Nuclei have been counterstained with haematoxylin (blue).
Indirect immunostaining of PFA fixed brain section from a wild-type mouse and a triple transgenic Alzheimer's disease mouse with rabbit anti-CD11b (HS-384 103, dilution 1:500; red). Nuclei have been visualized by DAPI staining (blue).
Indirect immunostaining of PFA fixed brain sections from SARS-CoV-2 infected K18-hACE2 transgenic mice in comparison to a non-infected control using the monoclonal anti-SARS-CoV-2 nucleocapsid antibody clone #4A8 (cat. no.: HS-452 011, dilution 1:1000; red). Nuclei have been visualized by DAPI staining (blue).
Courtesy:
The mice were housed and infected at the Helmholtz Center for Infection Research in cooperation with Prof. Kröger and Prof. Cicin-Sain.
Immunohistochemical staining of formalin-fixed paraffin embedded brain sections from K18-hACE2 transgenic mice infected with SARS-CoV-2 or non-infected control using the monoclonal anti-SARS-CoV-2 nucleocapsid antibody clone #53E2 (cat.no.: HS-452 111, dilution 1:1000; DAB). Nuclei have been counterstained with haematoxylin (blue).
Courtesy:
The mice were housed and infected at the Helmholtz Center for Infection Research in cooperation with Prof. Kröger and Prof. Cicin-Sain.
Immunohistochemical staining of formalin-fixed paraffin embedded brain sections from K18-hACE2 transgenic mice infected with SARS-CoV-2 or non-infected control using the biotinylated monoclonal anti-SARS-CoV-2 nucleocapsid antibody clone #53E2 (cat.no.: HS-452 111BT, dilution 1:1000; DAB). Nuclei have been counterstained with haematoxylin (blue).
Courtesy:
The mice were housed and infected at the Helmholtz Center for Infection Research in cooperation with Prof. Kröger and Prof. Cicin-Sain.
Indirect immunostaining of formalin-fixed paraffin embedded mouse spleen section using rabbit monoclonal anti.mouse CD4 (cat. no.: HS-360 108, dilution 1:100; green) and rat anti-mouse CD3e (cat. no.: HS-431 117, dilution 1:100; red). CD3e+/CD4+ double positive cells (T helper cells) appear yellow. Nuclei have been visualized by DAPI staining (blue).
Immunohistochemical staining of formalin fixed paraffin-embedded mouse liver section with monoclonal rabbit anti-CD3e (cat. no.: HS-413 108, dilution 1:100; DAB). Nuclei have been counterstained with haematoxylin (blue).
Indirect immunstaining of formalin fixed paraffin-embedded mouse spleen section using rabbit monoclonal anti-mouse CD8a (cat. no.: HS-361 008, dilution 1:100; green) and rat monoclonal anti-mouse CD4 (cat. no. : HS-360 117, dilution 1:100 red). Nuclei have been visualized by DAPI staining.
F4/80 positive Kupffer cells in FFPE mouse liver. Immunohistochemical staining of a formalin fixed paraffin embedded mouse liver tissue section with monoclonal rabbit anti-F4/80 (cat, no. HS-397 008, dilution 1:100, DAB). Nuclei have been counterstained with haematoxylin (blue).
Indirect immunostaining of a formalin fixed paraffin embedded wild-type mouse spleen section (left) and identical treated section of a mouse spleen engrafted with human CD34+-cells (right; kindly provided by Charles River Discovery Research Services Germany GmbH, Freiburg, Germany) unsing rabbit anti-mouse Ki67 (cat. no. HS-398 108, dilution 1:500; green) and rat anti-human Lamin B1 (cat. no. HS-404 017, dilution 1:100; red). Nuclei have been visualized by DAPI stainin (blue).
