|Cat. No. HS-234 004||100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.|
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 500 gallery
|Immunogen||Synthetic peptide corresponding to AA 134 to 147 from rat IBA1 (UniProt Id: P55009)|
Reacts with: mouse (Q9EQW9), rat (P55009), human (P55008).
Other species not tested yet.
|Specificity||Specific for IBA 1. K.O.|
IBA 1 positive cells in FFPE mouse thymus section
Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).