Cat. No.: HS-375 017
Amount: 100 µg
Price:
$415.00
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Cat. No. HS-375 017 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 100 gallery Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-Fr: 1 : 500 (see remarks) gallery |
Clone | SY-322C12 |
Subtype | IgG2b (κ light chain) |
Immunogen | synthetic peptide corresponding to residues surrounding AA 847 of mouse CD11c (UniProt Id: Q9QXH4) |
Reactivity |
Reacts with: mouse (Q9QXH4). No signal: rat, human (P20702). Other species not tested yet. |
Specificity | Specific for mouse CD11c without cross-reactivity to human CD11c |
Remarks |
IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings |
Data sheet | hs-375_017.pdf |
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CD11c positive cells in FFPE mouse ileum section