Nucleocapsid CoV-1/2 antibody - HS-452 111BT

CoV2 protein N packages the viral genome RNA
Mouse monoclonal purified IgG
Cat. No.: HS-452 111BT
Amount: 100 µg
Price: $465.00
Cat. No. HS-452 111BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: 1 : 500 gallery  
ELISA: yes (see remarks) gallery  
Label biotin
Clone 53E2
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 419 from SARS-Cov-2 Nucleocapsid protein (UniProt Id: P0DTC9)
Epitop Epitop: AA 401 to 410 from SARS-Cov-2 Nucleocapsid protein (UniProt Id: P0DTC9)
Specificity Specific for nucleocapsid from SARS-CoV1 and SARS-CoV2

ELISA: This antibody is suitable as detector antibody for sandwich-ELISA with cat. no. HS-452 011 as capture antibody.

Data sheet hs-452_111bt.pdf
Cat. No.: HS-452 111BT
Amount: 100 µg
Price: $465.00

Detection of SARS-CoV-2 nucleocapsid protein in the brain of a SARS-CoV-2 infected K18-hACE2 transgenic mouse.


The severe acute respiratory syndrome coronavirus type 2 (SARS-CoV2) is an enveloped positive-sense single stranded RNA virus that has been identified in the beginning of 2020 (1). It infects human host cells by docking via its spike protein (S) to the ACE2 surface receptor (2) and can cause mild to very severe and even deadly Covid-19 courses (3).
The very abundant Nucleocapsid or N-protein packages the viral RNA (4) and shares only little homology to other abundant members of the coronavirus family like NL63, 229E, HKU1 or OC43. This characteristic makes it a suitable target to discriminate between Covid-19 and other Coronavirus infections.

Protocols for HS-452 111BT