|Cat. No. HS-452 111BT||100 µg purified IgG, lyophilized, biotin-labeled. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
IHC-P/FFPE: 1 : 500 gallery
ELISA: yes (see remarks) gallery
|Subtype||IgG2a (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 419 from SARS-Cov-2 Nucleocapsid protein (UniProt Id: P0DTC9)|
Epitop: AA 401 to 410 from SARS-Cov-2 Nucleocapsid protein (UniProt Id: P0DTC9)
|Specificity||Specific for SARS-CoV1 and SARS-CoV2|
ELISA: This antibody is suitable as detector antibody for sandwich-ELISA with cat. no. HS-452 011 as capture antibody.
Detection of SARS-CoV-2 nucleocapsid protein in the brain of a SARS-CoV-2 infected K18-hACE2 transgenic mouse.
The severe acute respiratory syndrome coronavirus type 2 (SARS-CoV2) is an enveloped positive-sense single stranded RNA virus that has been identified in the beginning of 2020 (1). It infects human host cells by docking via its spike protein (S) to the ACE2 surface receptor (2) and can cause mild to very severe and even deadly Covid-19 courses (3).
The very abundant Nucleocapsid or N-protein packages the viral RNA (4) and shares only little homology to other abundant members of the coronavirus family like NL63, 229E, HKU1 or OC43. This characteristic makes it a suitable target to discriminate between Covid-19 and other Coronavirus infections.